Non-Radioisotopic Differential Display Method to Directly Visualize and Amplify Differential Bands on Nylon Membrane
نویسندگان
چکیده
منابع مشابه
Non-radioisotopic differential display method to directly visualize and amplify differential bands on nylon membrane.
Differential display is a breakthrough in gene isolation methods (1,2). Compared with other gene isolation methods, it is powerful and relatively rapid. The method achieves isolation of differentially expressed genes by using four 3′-end degenerate primers and 20–40 5′-end arbitrary primers to perform RT–PCR. The PCR products are separated on a DNA sequencing gel and the differences between two...
متن کاملDifferential Transform Method to two-dimensional non-linear wave equation
In this paper, an analytic solution is presented using differential transform method (DTM) for a class of wave equation. The emphasis is on the nonlinear two-dimensional wave equation. The procedures introduced in this paper are in recursive forms which can be used to obtain the closed form of the solutions, if they are required. The method is tested on various examples, and the results reveal ...
متن کاملthe differential effects of debate and media analysis as relate to enhancing efl learners critical thinking ability and writing performances
critical thinking ability has an important role in education. accordingly, scholars around the world are searching for new ways for teaching and improving students critical thinking ability. in line with the studies in efl contexts supporting the positive relationship between critical thinking and writing performances, this study investigated the differential effects of halverson’s critical thi...
15 صفحه اولglobal results on some nonlinear partial differential equations for direct and inverse problems
در این رساله به بررسی رفتار جواب های رده ای از معادلات دیفرانسیل با مشتقات جزیی در دامنه های کراندار می پردازیم . این معادلات به فرم نیم-خطی و غیر خطی برای مسایل مستقیم و معکوس مورد مطالعه قرار می گیرند . به ویژه، تاثیر شرایط مختلف فیزیکی را در مساله، نظیر وجود موانع و منابع، پراکندگی و چسبندگی در معادلات موج و گرما بررسی می کنیم و به دنبال شرایطی می گردیم که متضمن وجود سراسری یا عدم وجود سراسر...
Differential Display Method Using Chemi - luminescent Detection
the LCLINK-D assembly. The sluggishness of the VHICH1-LINK-D reaction may be caused by formation of strong secondary structures within the coding region of CHI, which would also account for the relatively weaker amplification of the Fd gene (as compared to the VH gene) as observed previously (9). Although the PCR assembly strategy has been described as somewhat difficult (4), we find it an attr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1996
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/24.4.793